Difference between revisions of "Part:BBa K642007:Design"

(Design Notes)
(Source)
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===Source===
 
===Source===
  
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal10/1 promoter. The Gal10/1 promoter is commonly used in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.
+
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal10/1 promoter. The Gal10/1 promoter is commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.
  
 
===References===
 
===References===

Revision as of 17:28, 24 October 2011

Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 89
    Illegal BsaI.rc site found at 1379


Design Notes

The yeGFP part (BBa_K319039) from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon.

Source

The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal10/1 promoter. The Gal10/1 promoter is commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.

References