Difference between revisions of "Part:BBa K642007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The yeGFP part from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon to the reverse primer. | + | The yeGFP part (BBa_K319039) from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon to the reverse primer. |
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===Source=== | ===Source=== | ||
Revision as of 17:28, 24 October 2011
Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 89
Illegal BsaI.rc site found at 1379
Design Notes
The yeGFP part (BBa_K319039) from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon to the reverse primer.
Source
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal10/1 promoter. The Gal10/1 promoter is commonly used in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.