Difference between revisions of "Part:BBa K606063:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible. | + | To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible. Our choice is 2 mutations Amber. |
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===Source=== | ===Source=== | ||
Revision as of 17:07, 23 October 2011
T7 amber expression monitored by a linear system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 917
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 693
Design Notes
To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible. Our choice is 2 mutations Amber.
Source
The T7 amber polymerase is from the classic T7 polymerase (part BBa_K145001).