Difference between revisions of "Part:BBa K510047"
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'''pUC18Sfi''' is a pUC18-derived cloning vector harboring an ampicillin resistance marker, a high copy number '''mutant pMB1 replication origin''' and the pUC18 multi-cloning site flanked by two SfiI restriction sites. Because of its high copy number and its ability to replicate in any E. coli strain and in other enterobacteria, pUC18Sfi is suitable for '''maintenance''' of miniTn7 transposon derivatives, and for simple plasmid preparation and '''genetic manipulation'''. pUC18Sfi can be used to '''deliver transposons''' by transformation in bacteria in which it does not replicate (i.e., non-enteric bacteria), but not in the enterics. | '''pUC18Sfi''' is a pUC18-derived cloning vector harboring an ampicillin resistance marker, a high copy number '''mutant pMB1 replication origin''' and the pUC18 multi-cloning site flanked by two SfiI restriction sites. Because of its high copy number and its ability to replicate in any E. coli strain and in other enterobacteria, pUC18Sfi is suitable for '''maintenance''' of miniTn7 transposon derivatives, and for simple plasmid preparation and '''genetic manipulation'''. pUC18Sfi can be used to '''deliver transposons''' by transformation in bacteria in which it does not replicate (i.e., non-enteric bacteria), but not in the enterics. | ||
| + | [[Image:UPOSevilla2011-mini-Tn7-Gm-attTn7.png|700px|center]] | ||
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Latest revision as of 16:06, 22 October 2011
pUC18Sfi-miniTn7BB-Gm-attTn7
pUC18Sfi-miniTn7BB-Gm-attTn7 is a vehicle vector for the minitransposon miniTn7BB-Gm-attTn7. This transposon is a part of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacterial genomes.
The mini-Tn7-Gm-attTn7 artificial transposon has been constructed based on pUC18Sfi-miniTn7-Gm (BBa_K510000) by inserting a "portable attTn7" (BBa_K510022) in its BioBrick cloning site (BCS). This plasmid allows the integration of BioBricks into microbial chromosomes, using prefix or suffix cloning sites, without removing the attachment site of the Tn7 transposon (attTn7). This fact makes posible to integrate another BioBrick into the chromosome of a strain in which miniTn7BB-Gm-attTn7 transposition has occurred previously.
The structure of the miniTn7BB-Gm-attTn7 transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The portable attTn7 has been inserted in the BioBrick cloning site (BCS).
pUC18Sfi is a pUC18-derived cloning vector harboring an ampicillin resistance marker, a high copy number mutant pMB1 replication origin and the pUC18 multi-cloning site flanked by two SfiI restriction sites. Because of its high copy number and its ability to replicate in any E. coli strain and in other enterobacteria, pUC18Sfi is suitable for maintenance of miniTn7 transposon derivatives, and for simple plasmid preparation and genetic manipulation. pUC18Sfi can be used to deliver transposons by transformation in bacteria in which it does not replicate (i.e., non-enteric bacteria), but not in the enterics.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4367
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4373 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4367
Illegal BglII site found at 3051
Illegal BglII site found at 3322
Illegal BglII site found at 3608 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4367
Illegal XbaI site found at 4382
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1681
Illegal SapI.rc site found at 2763