Difference between revisions of "Part:BBa K510046"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K510046 short</partinfo>
 
<partinfo>BBa_K510046 short</partinfo>
  
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This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K51019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51012) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
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This plasmid allows the integration of the improved flip-flop (module I) (part: [https://parts.igem.org/Part:BBa_K510019 BBa_K51019]) into bacterial chromosomes using the pUC18R6KT-miniTn7BB-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K51012]) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
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For more information about the function of the miniTn7 BioBrick toolkit and flip-flops, visit the iGEM team UPO-Sevilla 2011 wiki.
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For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] and [http://2011.igem.org/Team:UPO-Sevilla/Project flip-flops], visit the iGEM team UPO-Sevilla 2011 wiki.
  
  

Revision as of 11:09, 22 October 2011

pUC18R6KT-miniTn7BB-Gm-improved_flipflop_(module I)

This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K51019) into bacterial chromosomes using the pUC18R6KT-miniTn7BB-Gm (BBa_K51012) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.

For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit] and [http://2011.igem.org/Team:UPO-Sevilla/Project flip-flops], visit the iGEM team UPO-Sevilla 2011 wiki.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal NheI site found at 7687
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BamHI site found at 5497
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 8949
    Illegal AgeI site found at 9061
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4828
    Illegal BsaI.rc site found at 1854
    Illegal SapI site found at 518