Difference between revisions of "Part:BBa K510045"

Revision as of 10:54, 22 October 2011

pUC18Sfi-miniTn7BB-Gm-improved_flipflop_(module I)

This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K51019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51000) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.

For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 4367
Illegal NheI site found at 7526
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4373
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 4367
Illegal BglII site found at 3051
Illegal BglII site found at 3322
Illegal BglII site found at 3608
Illegal BamHI site found at 5336
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 4367
Illegal XbaI site found at 4382
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 8788
Illegal AgeI site found at 8900
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1681
Illegal BsaI site found at 4667
Illegal SapI.rc site found at 2763

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K510045 short</partinfo>
-This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K510019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51000) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
-For more information about the function of the miniTn7 BioBrick toolkit, visit the iGEM team UPO-Sevilla 2011 wiki.
+This plasmid allows the integration of the improved flip-flop (module I) (part: [https://parts.igem.org/Part:BBa_K510019 BBa_K51019]) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm ([https://parts.igem.org/Part:BBa_K510000 BBa_K51000]) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
+For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.
 <!-- Add more about the biology of this part here