Difference between revisions of "Part:BBa K510044"

Revision as of 18:46, 21 October 2011

pUC18R6KT-miniTn7BB-Gm-bistable_switch

This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the pUC18R6KT-miniTn7BB-Gm (BBa_K510012)characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.

For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 4528
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
Illegal BglII site found at 7615
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 8202
Illegal AgeI site found at 8314
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1854
Illegal BsaI.rc site found at 6171
Illegal SapI site found at 518

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K510044 short</partinfo>
-This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the miniTn7BB characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
-For more information about the function of the miniTn7 BioBrick toolkit, visit the page of the pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012).
+This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the pUC18R6KT-miniTn7BB-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012])characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
+For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.