Difference between revisions of "Part:BBa K633000"
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CelD Mutated Cellulase
 
  
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Clostridium thermocellum endoglucanase CelD was chosen as a representative member for detailedstructural and functional studies of the family E cellulases. Beside C. thermocellum’s CelD, E family cellulases include a number of cellulases of bacterial, fungal, and plant origin.  
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Clostridium thermocellum endoglucanase CelD was chosen as a representative member for detailedstructural and functional studies of the family E cellulases.  
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Beside C. thermocellum’s CelD, E family cellulases include a number of cellulases of bacterial, fungal, and plant origin.  
 
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CelD, has been previously overexpressed in Escherichia coli, the enzyme is easily purified in large amounts from cytoplasmic inclusion bodies. (Chauvaux, Beguin, Aubert, 1992)
 
CelD, has been previously overexpressed in Escherichia coli, the enzyme is easily purified in large amounts from cytoplasmic inclusion bodies. (Chauvaux, Beguin, Aubert, 1992)
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The CelD sequence used has an aminoacid substittition Asp-523 ---> Ala. That mutation increases the specific activity of the enzyme by 224% (Chauvaux, Beguin, Aubert, 1992)
 
The CelD sequence used has an aminoacid substittition Asp-523 ---> Ala. That mutation increases the specific activity of the enzyme by 224% (Chauvaux, Beguin, Aubert, 1992)
 
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[[Image:Celd_chart.png]]__NOTOC__
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<center>[[Image:Celd_chart.png]]__NOTOC__</center>
 
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Revision as of 01:01, 19 October 2011

CelD Mutated Cellulase




Clostridium thermocellum endoglucanase CelD was chosen as a representative member for detailedstructural and functional studies of the family E cellulases.
Beside C. thermocellum’s CelD, E family cellulases include a number of cellulases of bacterial, fungal, and plant origin.
CelD, has been previously overexpressed in Escherichia coli, the enzyme is easily purified in large amounts from cytoplasmic inclusion bodies. (Chauvaux, Beguin, Aubert, 1992)
The CelD sequence used has an aminoacid substittition Asp-523 ---> Ala. That mutation increases the specific activity of the enzyme by 224% (Chauvaux, Beguin, Aubert, 1992)

Celd chart.png



Chauvaux, S., Beguin, P., & Aubert, J. (1992). Site-directed mutagenesis of essential carboxylic residues in clostridium thermocellum endoglucanase celd*. The Journal of Biological Chemistry, 267(5), 4472-4478. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 508
    Illegal AgeI site found at 151
    Illegal AgeI site found at 567
    Illegal AgeI site found at 1396
  • 1000
    COMPATIBLE WITH RFC[1000]