Difference between revisions of "Part:BBa J176019:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
PCR-cloned from the pNEB plasmid, using the following primers:<br>
 
PCR-cloned from the pNEB plasmid, using the following primers:<br>
−
Forward: 5'-<ul>CCTTTCTAGA</ul>CGGAGTACTGTCCTCCGAGC<br>
+
Forward: 5'-<u>CCTTTCTAGA</u>CGGAGTACTGTCCTCCGAGC<br>
−
Reverse: 5'-<ul>AAGGCTGCAGCGGCCGCTACTAGT</ul>CGGAGGACAGTACTCCGCTC<br>
+
Reverse: 5'-<u>AAGGCTGCAGCGGCCGCTACTAGT</u>CGGAGGACAGTACTCCGCTC<br>
  
 
These primers add a XbaI site upstream of the part and SpeI, NotI, and PstI sites downstream. The PCR amplicon was digested with XbaI/PstI and inserted into an empty V0120 vector.
 
These primers add a XbaI site upstream of the part and SpeI, NotI, and PstI sites downstream. The PCR amplicon was digested with XbaI/PstI and inserted into an empty V0120 vector.

Revision as of 02:59, 16 October 2011

5xGal4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR-cloned from the pNEB plasmid, using the following primers:
Forward: 5'-CCTTTCTAGACGGAGTACTGTCCTCCGAGC
Reverse: 5'-AAGGCTGCAGCGGCCGCTACTAGTCGGAGGACAGTACTCCGCTC

These primers add a XbaI site upstream of the part and SpeI, NotI, and PstI sites downstream. The PCR amplicon was digested with XbaI/PstI and inserted into an empty V0120 vector.

Source

TBA

References