Difference between revisions of "Part:BBa K658014:Design"

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===References===
 
===References===
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[1] Baldwin T, Devine JH, Heckel, RC, Lin, JW, Shadel GS. The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence[J]. Journal of Bioluminescence and Chemiluminescence, 1989, 4(1): 326-341.
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[2] Koch B,  Liljefors T,  Persson T , Nielsen J,  Kjelleberg S, Givskov M. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors[J].Microbiology , 2005, 151 (11): 3589-3602.
 +
 +
[3] Kumari A, Pasini P, Deo S. K., Flomenhoft D, Shashidhar S, Daunert S. Biosensing Systems for the Detection of Bacterial Quorum Signaling Molecules[J]. Analytical Chemistry,2005, 78 (22): 7603–7609.
 +
 +
[4] Eberhard A, Burlingame A. L, Eberhard C, Kenyon G. L, Nealson K. H,Oppenheimer N. J. Structural identification of autoinducer of Photobacterium fischeri luciferase[J]. Biochemistry ,1981,20 (9): 2444–2449.
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[5] http://scholarworks.umass.edu/dissertations/AAI3397713/
 +
 +
[6] Gerdes K. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress[J]. J. Bacteriol,2000,182 (3): 561–721.
 +
 +
[7]Philippe B,Martine C.Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes[J].Molecular Biology,1992,226(3):735-745.
 +
 +
[8]Devine J.H,Shadel G.S,Baldwin T.O. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. Proceedings of the National Academy of Sciences.1989.86(15):5688-5692.
 +
 +
[9] http://www.uniprot.org/uniprot/P12747

Latest revision as of 09:36, 15 October 2011

3OC6HSL Sender and Receiver Device with lux pR-5 Driven by PLlac 0-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1874


Design Notes

This signalling device is based on the quorum sensing system in V. Fischri. Different from an autoinduction system, this signalling circuit starts only at the presence of inducer IPTG which does not exist in the natural QS system.


Source

F1610 K658000 K658012

References

[1] Baldwin T, Devine JH, Heckel, RC, Lin, JW, Shadel GS. The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence[J]. Journal of Bioluminescence and Chemiluminescence, 1989, 4(1): 326-341.

[2] Koch B, Liljefors T, Persson T , Nielsen J, Kjelleberg S, Givskov M. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors[J].Microbiology , 2005, 151 (11): 3589-3602.

[3] Kumari A, Pasini P, Deo S. K., Flomenhoft D, Shashidhar S, Daunert S. Biosensing Systems for the Detection of Bacterial Quorum Signaling Molecules[J]. Analytical Chemistry,2005, 78 (22): 7603–7609.

[4] Eberhard A, Burlingame A. L, Eberhard C, Kenyon G. L, Nealson K. H,Oppenheimer N. J. Structural identification of autoinducer of Photobacterium fischeri luciferase[J]. Biochemistry ,1981,20 (9): 2444–2449.

[5] http://scholarworks.umass.edu/dissertations/AAI3397713/

[6] Gerdes K. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress[J]. J. Bacteriol,2000,182 (3): 561–721.

[7]Philippe B,Martine C.Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes[J].Molecular Biology,1992,226(3):735-745.

[8]Devine J.H,Shadel G.S,Baldwin T.O. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. Proceedings of the National Academy of Sciences.1989.86(15):5688-5692.

[9] http://www.uniprot.org/uniprot/P12747