Difference between revisions of "Part:BBa K658006:Design"

(→‎Design Notes)
(→‎References)
Line 13: Line 13:
  
 
===References===
 
===References===
 +
[1]Baldwin T, Devine JH, Heckel, RC, Lin, JW, Shadel GS. The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence[J]. Journal of Bioluminescence and Chemiluminescence, 1989, 4(1): 326-341.

Revision as of 09:20, 15 October 2011

position 3 mutated promoter lux pR-3 (luxR & HSL regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By PCR method the C residue of position 3 was changed to a T based on the sequence of the promoter lux pR(BBa_R0062).

Source

The position 3 mutated promoter lux pR-3 is based on promoter lux pR (BBa_R0062).

References

[1]Baldwin T, Devine JH, Heckel, RC, Lin, JW, Shadel GS. The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescence[J]. Journal of Bioluminescence and Chemiluminescence, 1989, 4(1): 326-341.