Difference between revisions of "Part:BBa K559001"

 
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__NOTOC__
 
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<partinfo>BBa_K559001 short</partinfo>
 
<partinfo>BBa_K559001 short</partinfo>
  
 
Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
 
Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
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The reason of using T7 promoter is providing unlimited expression of the downstream halorhodopsin gene, so that the halorhodopsin light-driven ion pump will not be a limiting factor in the light sensing system.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:32, 14 October 2011

Halorhodopsin under T7 promoter

Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor The reason of using T7 promoter is providing unlimited expression of the downstream halorhodopsin gene, so that the halorhodopsin light-driven ion pump will not be a limiting factor in the light sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 316