Difference between revisions of "Part:BBa K624027"

Revision as of 19:58, 14 October 2011

pYMB (shuttle vector between Escherichia coli and Magnetospirillum magneticum AMB-1)

Shuttle vector between E. coli and Magnetospirillum magneticum AMB-1, containing [ori+Pmsp1+rep] (essentials of pYMB) on pUC19 pYMB(Fig. 1) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

Fig.1 pYMB Work Flow
The way we design the shuttle vector for magnetic bacteria

Sequence and Features