Difference between revisions of "Part:BBa K622006:Experience"

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Since DNS assay is known as a method to detect the amount of reducing sugar, this assay was used to measure the quantity of N-acetylglucosamine, the main product of decomposition of chitin.
 
Since DNS assay is known as a method to detect the amount of reducing sugar, this assay was used to measure the quantity of N-acetylglucosamine, the main product of decomposition of chitin.
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The chitinase activity was measured in the supernatant of culture. Figure 1 shows the amount of reducing sugar.
  
 
[[Image:Kyoto-ChitinaseAssay.png|500px|thumb|center|Fig.1:]]
 
[[Image:Kyoto-ChitinaseAssay.png|500px|thumb|center|Fig.1:]]

Revision as of 19:55, 14 October 2011

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Applications of BBa_K622006

The function check of chitinase activity was performed by using DNS assay.

Since DNS assay is known as a method to detect the amount of reducing sugar, this assay was used to measure the quantity of N-acetylglucosamine, the main product of decomposition of chitin.

The chitinase activity was measured in the supernatant of culture. Figure 1 shows the amount of reducing sugar.

Fig.1:

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