Difference between revisions of "Part:BBa K624002"

Latest revision as of 19:52, 14 October 2011

pT7+RBS+tetR+RBS+GFP+Ter

TetR and GFP genes are both under pT7 promoter control. However, pT7 can only function under presence of associated regulatory protein; thus, for example, when we transform this construct into strain BL21 E. coli, which express associated regulator, both tetR and GFP will be expressed.In contrast, if we transform this construct into other strain, such as DH5alpha and S17-1, both tetR and GFP genes will not be expressed. Due to that tetR and GFP are under the regulation of the same promoter, we can quantitize the tetR expression by detection intensity of GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1430

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 <partinfo>BBa_K624002 short</partinfo>
-TetR and GFP genes are both under pT7 promoter control. However, pT7 can only function uder the presence of associated refulator, thus, for example, when we transform this construct into strain BL21 E. coli, which express associated regulator, both tetR and GFP will be expressed.In contrast, if we transform this construct into other strain without regulatory protein both genes will not expressed. Due to that tetR and GFP is under sthe regulation of the same promoter, we can quantitize the tetR expression by detection of GFP.
+TetR and GFP genes are both under pT7 promoter control. However, pT7 can only function under presence of associated regulatory protein; thus, for example, when we transform this construct into strain BL21 E. coli, which express associated regulator, both tetR and GFP will be expressed.In contrast, if we transform this construct into other strain, such as DH5alpha and S17-1,  both tetR and GFP genes will not be expressed. Due to that tetR and GFP are under the regulation of the same promoter, we can quantitize the tetR expression by detection intensity of GFP.
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