Difference between revisions of "Part:BBa K624057"
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Revision as of 19:51, 14 October 2011
pYMB essentials + RBS(Pmsp3) + LLO
pYMB essentials + RBS(Pmsp3) + LLO
pYMB (BBa_K624004)is a shuttle vector for manipulating and transforming gene into AMB-1.
The ribosome binding site is from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012).
Listeriolysin O (LLO)is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2567
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
- 1000COMPATIBLE WITH RFC[1000]
