Difference between revisions of "Part:BBa K602013:Experience"
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In general, a high background level of lycopene was measured even in the absence of any irradiation. This might be due to basal expression from the SOS promoter which is needed for the SOS genes' rapid response to damage. Therefore, promoter response to UV irradiation was defined as the fractional increase of lycopene production over non-irradiated controls, for each irradiated sample/UV energy dosage. | In general, a high background level of lycopene was measured even in the absence of any irradiation. This might be due to basal expression from the SOS promoter which is needed for the SOS genes' rapid response to damage. Therefore, promoter response to UV irradiation was defined as the fractional increase of lycopene production over non-irradiated controls, for each irradiated sample/UV energy dosage. | ||
| − | [[Image:2011_osaka_promoter.png| | + | [[Image:2011_osaka_promoter.png|500px]] |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:27, 14 October 2011
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Applications of BBa_K602013
2011 Osaka
This part was assayed for DNA damage detection ability as follows. E. coli transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficient time for lycopene production. Following that, the lycopene was extracted using acetone. The lycopene concentration was measured by absorbance at 474 nm and regarded as an approximate indicator of promoter activity.
In general, a high background level of lycopene was measured even in the absence of any irradiation. This might be due to basal expression from the SOS promoter which is needed for the SOS genes' rapid response to damage. Therefore, promoter response to UV irradiation was defined as the fractional increase of lycopene production over non-irradiated controls, for each irradiated sample/UV energy dosage.
User Reviews
UNIQ8c16c65eb1098875-partinfo-00000000-QINU UNIQ8c16c65eb1098875-partinfo-00000001-QINU