Difference between revisions of "File:2011 osaka tolerance.png"
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| − | <i>D. radiodurans</i> proteins related to DNA damage repair (PprI, PprM, PprA, RecA) were assayed for their ability to confer damage tolerance to host <i>E. coli</i> cells. | + | <i>D. radiodurans</i> proteins related to DNA damage repair (PprI, PprM, PprA, RecA) were assayed for their ability to confer damage tolerance to host <i>E. coli</i> cells. Transformed and pre-cultured cells were plated and exposed to varying doses of UV radiation. Plates were then wrapped with aluminum foil to prevent further exposure and incubated for 16h. From colony counts, the survival percentages of irradiated samples relative to controls were calculated and used as an indicator of tolerance. |
Latest revision as of 19:04, 14 October 2011
D. radiodurans proteins related to DNA damage repair (PprI, PprM, PprA, RecA) were assayed for their ability to confer damage tolerance to host E. coli cells. Transformed and pre-cultured cells were plated and exposed to varying doses of UV radiation. Plates were then wrapped with aluminum foil to prevent further exposure and incubated for 16h. From colony counts, the survival percentages of irradiated samples relative to controls were calculated and used as an indicator of tolerance.
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| current | 19:01, 14 October 2011 |  | 1,319 × 653 (83 KB) | Shao (Talk | contribs) | D. radiodurans proteins related to DNA damage repair (PprI, PprM, PprA, RecA) were assayed for their ability to confer damage tolerance to host E. coli cells. Transformed and pre-cultured cells were plated and exposed to varying doses of UV radiation. P |
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