Difference between revisions of "File:2011 osaka promoter.png"
(We constructed a DNA damage detection device by attaching a lycopene biosynthesis gene cluster (crtE, B, I) downstream of the SOS promoter. E. coli transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficie) |
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| − | We constructed a DNA damage detection device by attaching a lycopene biosynthesis gene cluster (crtE, B, I) downstream of the SOS promoter. E. coli transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficient time for lycopene production. Following that, the lycopene was extracted using acetone. The lycopene concentration was measured by absorbance at 474 nm and regarded as an approximate indicator of promoter activity. | + | We constructed a DNA damage detection device by attaching a lycopene biosynthesis gene cluster (crtE, B, I) downstream of the SOS promoter. <i>E. coli</i> transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficient time for lycopene production. Following that, the lycopene was extracted using acetone. The lycopene concentration was measured by absorbance at 474 nm and regarded as an approximate indicator of promoter activity. |
Latest revision as of 19:04, 14 October 2011
We constructed a DNA damage detection device by attaching a lycopene biosynthesis gene cluster (crtE, B, I) downstream of the SOS promoter. E. coli transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficient time for lycopene production. Following that, the lycopene was extracted using acetone. The lycopene concentration was measured by absorbance at 474 nm and regarded as an approximate indicator of promoter activity.
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| current | 19:03, 14 October 2011 |  | 1,269 × 863 (86 KB) | Shao (Talk | contribs) | We constructed a DNA damage detection device by attaching a lycopene biosynthesis gene cluster (crtE, B, I) downstream of the SOS promoter. E. coli transformed with this part was irradiated with UV light, and then incubated for 2 hours to provide sufficie |
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