Difference between revisions of "Part:BBa K632001"

Latest revision as of 21:09, 13 October 2011

INP-GFP

This part contains a fusion of an existent ice nucleation gene (BBa_K265009) and a GFP gene. GFP has been successfully fused to INP, verified by gel electrophoresis and DNA sequencing. The size of the product is correct and the sequence suggests it is in the correct orientation and frame. However, the cells with the INP-GFP fusion gene would not fluorescent. Spectrometer readings suggest limited fluorescence. We believe this construct is correct, but it needs to be tested in a different plasmid under the control of another promoter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal PstI site found at 2113
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal PstI site found at 2113
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
Illegal BamHI site found at 337
Illegal XhoI site found at 1387
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal PstI site found at 2113
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal PstI site found at 2113
Illegal NgoMIV site found at 747
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:13, 9 October 2011 (view source) Dingx055 (Talk \| contribs) ← Older edit		Latest revision as of 21:09, 13 October 2011 (view source) Dingx055 (Talk \| contribs)
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	This part contains a fusion of an existent ice nucleation gene (BBa_K265009) and a GFP gene.		This part contains a fusion of an existent ice nucleation gene (BBa_K265009) and a GFP gene.
−	GFP has been successfully fused to INP, verified by gel electrophoresis and DNA sequencing. The size of the product is correct and the sequence suggests it is in the correct orientation and frame. However, the cells with the INP-~~SIL~~ fusion gene would not fluorescent. Spectrometer readings suggest limited fluorescence. We believe this construct is correct, but it needs to be tested in a different plasmid under the control of another promoter.	+	GFP has been successfully fused to INP, verified by gel electrophoresis and DNA sequencing. The size of the product is correct and the sequence suggests it is in the correct orientation and frame. However, the cells with the INP-GFP fusion gene would not fluorescent. Spectrometer readings suggest limited fluorescence. We believe this construct is correct, but it needs to be tested in a different plasmid under the control of another promoter.