Difference between revisions of "Part:BBa K633014"
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The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator | The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator | ||
| − | The construct efectiveness was tested by an enzymatic assay, The results can be seen here | + | The construct efectiveness was tested by an enzymatic assay, The results can be seen here: http://2011.igem.org/Team:Tec-Monterrey/projectresults |
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Revision as of 06:08, 12 October 2011
AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein
The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator
The construct efectiveness was tested by an enzymatic assay, The results can be seen here: http://2011.igem.org/Team:Tec-Monterrey/projectresults
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
Illegal NheI site found at 3168 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
Illegal BamHI site found at 1981 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1845
Illegal NgoMIV site found at 3398
Illegal AgeI site found at 979
Illegal AgeI site found at 1488
Illegal AgeI site found at 1904
Illegal AgeI site found at 2733 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961