Difference between revisions of "Part:BBa K609002:Experience"

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===Applications of BBa_K609002===
 
===Applications of BBa_K609002===
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<p>1.In order to test the biobrick, we ligate the part with ''nisI'', which is the part BBa_K609004, and pMG36e(for the convenience of screening in ''E.coli'' and the erythromycin resistance gene will be knocked off later). The figures below show the recombinant plasmid and the result of ligation separately.</p>
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<p>1.In order to test the biobrick, we ligate the part with ''nisI'', which is the part BBa_K609004, and the ''E.coli-Lactococcus lactis'' shuttle plasmid pMG36e(the erythromycin resistance gene will be knocked off later). The figures below show the recombinant plasmid and the result of ligation separately.</p>
 
<center>[[Image:J+N+pMG36e.jpg]]</center>
 
<center>[[Image:J+N+pMG36e.jpg]]</center>
  

Latest revision as of 06:13, 11 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K609002

1.In order to test the biobrick, we ligate the part with nisI, which is the part BBa_K609004, and the E.coli-Lactococcus lactis shuttle plasmid pMG36e(the erythromycin resistance gene will be knocked off later). The figures below show the recombinant plasmid and the result of ligation separately.

J+N+pMG36e.jpg


The restriction pattern of recombinant plasmid for producing collagen.jpg

2.To transduce the recombinant plasmid to S.thermophilus by the means of electroporation.

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