Difference between revisions of "Part:BBa K629013:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Latest revision as of 09:26, 10 October 2011
T7 promoter + lac operator + RBS + trkD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 61
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 346
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 61
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 61
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1421
Design Notes
the distance from RBS to ATG is 9bp.
Source
pET-32a, genome of E. Coli Bl21 Plys