Difference between revisions of "Part:BBa K676012:Design"

Latest revision as of 02:20, 10 October 2011

Gyrase Binding Site from pBR322

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 149
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Performed PCR to clone out the GBS from the pBR322 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.

Source

pBR322 Plasmid DNA

References

Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347

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	===References===		===References===
		+	Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347