Difference between revisions of "Part:BBa K676012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Performed PCR to clone out the GBS from the pBR322 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone. | |
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===Source=== | ===Source=== | ||
Revision as of 02:19, 10 October 2011
Gyrase Binding Site from pBR322
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 149
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Performed PCR to clone out the GBS from the pBR322 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
Source
PCR and then 2 parts ligation.