Difference between revisions of "Part:pSB1C3:Experience"
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| − | <!-- | + | <!-- The 2011 Nevada iGEM Team attempted to use pSB1C3 as a template to amplify the chloramphenicol resistance cassette for use in a new part. When they attempted to design primers for this purpose, we were unable to identify a start codon. We subsequently contacted Dr. Knight and Austin Che about the problem. They re-examined the sequence and found that the sequence in the registry was missing two nucleotides which resulted in an apparent frameshift. Upon fixing the sequence annotation, we were able to design our amplification primers for the chloramphenicol cassette. <br> |
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Revision as of 23:25, 9 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of pSB1C3
User Reviews
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UNIPV-Pavia iGEM 2011 |
This cloning vector has been improved, cloning in it the strong pTet promoter, between E and X restriction sites; the mRFP coding sequence from BBa_J61002, has been placed between S and P restirction sites, in order to facilitate the cloning of coding sequences downstream pTet promoter in this high copy number plasmid. |
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