Difference between revisions of "Part:BBa K631032"
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Composite part designed to modify chemotaxis of C. elegans. <P> | Composite part designed to modify chemotaxis of C. elegans. <P> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 23:06, 9 October 2011
ODR7:ADBR2:UNC-54 (for use in C. elegans)
Composite part designed to modify chemotaxis of C. elegans.
Characterization
Part was characterized through the use of a chemotaxis assay.
Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant.
Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. <p>
Result
The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1308
Illegal BamHI site found at 2661 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1873