Difference between revisions of "Part:BBa K631032"

Revision as of 23:03, 9 October 2011

ODR7:ADBR2:UNC-54 (for use in C. elegans)

Composite part designed to modify chemotaxis of C. elegans.

Characterization

Part was characterized through the use of a chemotaxis assay. Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. Result

The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1308
Illegal BamHI site found at 2661
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1873

@@ Line 5: / Line 5: @@
 <b>Characterization</b><p>
 Part was characterized through the use of a chemotaxis assay.
 Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant.
@@ Line 12: / Line 13: @@
-<b>Result</b><p>
+<b>Result</b> <html> <p> </html>
 The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below.
 [[Image:Queens_CanadaGraph.png|thumb|569px|center]]