Difference between revisions of "Part:BBa K631032"
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Composite part designed to modify chemotaxis of C. elegans. | Composite part designed to modify chemotaxis of C. elegans. | ||
| − | <b>Characterization</b> | + | <b>Characterization</b><p> |
Part was characterized through the use of a chemotaxis assay. | Part was characterized through the use of a chemotaxis assay. | ||
Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. | Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. | ||
| − | + | <p> | |
Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. | Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. | ||
| + | <p> | ||
| − | + | <b>Result</b><p> | |
| − | <b>Result</b> | + | |
The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below. | The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below. | ||
[[Image:Queens_CanadaGraph.png|thumb|569px|center]] | [[Image:Queens_CanadaGraph.png|thumb|569px|center]] | ||
Revision as of 22:55, 9 October 2011
ODR7:ADBR2:UNC-54 (for use in C. elegans)
Composite part designed to modify chemotaxis of C. elegans.
CharacterizationPart was characterized through the use of a chemotaxis assay. Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. <p> Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. <p> Result<p> The results of the chemotaxis assay for the ODR-7:ADBR2:UNC-54 part are shown below.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1308
Illegal BamHI site found at 2661 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1873