Difference between revisions of "Part:BBa K676001"
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By introducing the Mu GBS into the target plasmids, we managed to achiev a higher level of supercoiling as well as better uniformity in the final supercoiled products. Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as for the delivery therapy of therapeutic genes. | By introducing the Mu GBS into the target plasmids, we managed to achiev a higher level of supercoiling as well as better uniformity in the final supercoiled products. Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as for the delivery therapy of therapeutic genes. | ||
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Revision as of 22:29, 9 October 2011
Gyrase Binding Site from Mu Bacteriophage
The strong gyrase site cloned from Mu bacteriophage genome.
Usage and Biology
The Mu phage string gyrase site (SGS) serves as a high affinity binding site for the DNA gyrase enzyme. This site is known to be cleaved preferentially by the Gyrase A subunits and therefore results in an increase in the linking number difference of the DNA molecule.
Apparently, some bacteriophages are known to exploit the GBS to allow them to replicate successfully in their host. For example, Mu bacteriophage has a strong gyrase site or GBS in the central region of their 37.2 kbp genome. From work carried out by Pato et al. in 1990, it was found out that the wild type phage can carry out replication effectively compared a mutant which lacks the GBS after the heat induction of the lysogens.
By introducing the Mu GBS into the target plasmids, we managed to achiev a higher level of supercoiling as well as better uniformity in the final supercoiled products. Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as for the delivery therapy of therapeutic genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000COMPATIBLE WITH RFC[1000]