Difference between revisions of "Part:BBa K624023"

 
 
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__NOTOC__
 
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<partinfo>BBa_K624023 short</partinfo>
 
<partinfo>BBa_K624023 short</partinfo>
  
 
Positive control for BRET. This also serve to examine the effects of N-terminal fusion on correct folding of mms13 (the "13" indicated in the name).
 
Positive control for BRET. This also serve to examine the effects of N-terminal fusion on correct folding of mms13 (the "13" indicated in the name).
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[[Image: c4_NYMU.png|frame|none|Fig. 6: This design is constructed with the whole YFP on the N-terminus of Mms13 but “without” the CHAMP design. The construct should be a positive control with constant light no matter whether force is applied or not.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:03, 9 October 2011

pYMB essentials+[Y-13-R]

Positive control for BRET. This also serve to examine the effects of N-terminal fusion on correct folding of mms13 (the "13" indicated in the name).

Fig. 6: This design is constructed with the whole YFP on the N-terminus of Mms13 but “without” the CHAMP design. The construct should be a positive control with constant light no matter whether force is applied or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 2034
    Illegal NgoMIV site found at 2118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2784