Difference between revisions of "Part:BBa K624022"

Latest revision as of 13:01, 9 October 2011

pYMBG (rbs: trunc. Pmsp3) essentials

pYMB is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K624022 short</partinfo>
+*pYMB is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
-pYMB indicates "ori+Pmsp1+rep on pUC19", where GFP with 6 pbs-truncated rbs from Pmsp3 locates downstream.
+*pYMB with GFP and 6 bps-truncated rbs from Pmsp3 locates downstream.
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