Difference between revisions of "Part:BBa K624021"

 
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<partinfo>BBa_K624021 short</partinfo>
 
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pYMB is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.</font>
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[[Image: PYMBWorkflow.jpg|center|frame|<center>Fig.1 pYMB Work Flow <br>The way we design the shuttle vector for magnetic bacteria</center>]]
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In this part, pYMB with GFP and rbs from Pmsp3 locates downstream.
  
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pYMB indicates "ori+Pmsp1+rep on pUC19", where GFP with rbs from Pmsp3 locates downstream.
 
  
 
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Revision as of 12:58, 9 October 2011

pYMBG (rbs: Pmsp3) essentials pYMB is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.</font>

Fig.1 pYMB Work Flow
The way we design the shuttle vector for magnetic bacteria

In this part, pYMB with GFP and rbs from Pmsp3 locates downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1831