Difference between revisions of "Part:BBa K624044"

Revision as of 12:57, 9 October 2011

pYMB essentials + RBS + LLO + RBS + Invasin + RBS + ECFP

ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP

pYMB (BBa_K624004) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2567
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3573
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 4190
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP
-The ori serve as the Origin of Replication,and
+pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
-the Pmsp1 is the second strongest promoter
-on AMB-1.
+Listeriolysin O (LLO) is a hemolysin produced by the bacterium ''Listeria monocytogenes'', a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed ''L. monocytogenes'' cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
-LLO (Listeriolysin O) can make the bacteria be able to
-escape into the cytosol without damaging the plasma
-membrane, and grow intracellularly.
-which help protect the AMB-1 from extracellular immune system.
-ECFP serves as a reporter to show if the construct work or not.
 <!-- Add more about the biology of this part here