Difference between revisions of "Part:BBa K624020"
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negative control aimed at examining the folding state and location of 13-R with simple C-terminal rLuc fusion | negative control aimed at examining the folding state and location of 13-R with simple C-terminal rLuc fusion | ||
| + | [[Image: c5_NYMU.png|frame|none|Fig. 7: The design construct is similar with construct in Fig. 5. Why we construct this design with the whole YFP on the C-terminus of the CHAMP design instead of Mms13’s N-terminus is that we try to avoid the interferences of DNA transformation with sequences added in front of the natural construct. The construct is supposed to light up when force pull the Mms13 and turn off if no magnetic field is applied.]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 12:51, 9 October 2011
pYMB essentials+[13-R]
negative control aimed at examining the folding state and location of 13-R with simple C-terminal rLuc fusion
Fig. 7: The design construct is similar with construct in Fig. 5. Why we construct this design with the whole YFP on the C-terminus of the CHAMP design instead of Mms13’s N-terminus is that we try to avoid the interferences of DNA transformation with sequences added in front of the natural construct. The construct is supposed to light up when force pull the Mms13 and turn off if no magnetic field is applied.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 1809
Illegal NgoMIV site found at 1893 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2559