Difference between revisions of "Part:BBa K624051"

Revision as of 12:35, 9 October 2011

pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP

pYMB (BBa_K624004) is a shuttle vector for manipulating and transforming gene into AMB-1.

Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

All of the ribosome binding sites are from Psmp3 with 6 bps truncated.(BBa_K624013).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2558
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3555
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 4172
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K624051 short</partinfo>
-RBS(trunc.) + minC + mcherry
+pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP
+pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is a shuttle vector for manipulating and transforming gene into AMB-1.
+Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
+Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
+All of the ribosome binding sites are from Psmp3 with 6 bps truncated.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]).
 <!-- Add more about the biology of this part here