Difference between revisions of "Part:BBa K624052"

Revision as of 12:28, 9 October 2011

Pmsp1(tetO) + RBS(trunc.) + minC + RBS(trunc.) + mcherry

pYMB essentials + RBS(trunc.) + minC + RBS(trunc.) + mcherry

pYMB (BBa_K624004) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

All of the ribosome binding sites are RBS from Pmsp3 with 6 bps truncated(BBa_K624013).

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features