Difference between revisions of "Part:BBa K624041"

Revision as of 12:08, 9 October 2011

pT7-tetO+RBS+minC+mcherry

This construct is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter. Another construct which regulates this construct is pT7+RBS+tetR+RBS+GFP+Ter (BBa_K624002).

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 259
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K624041 short</partinfo>
-This part is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter.
+This construct is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter.
+Another construct which regulates this construct is pT7+RBS+tetR+RBS+GFP+Ter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624002 BBa_K624002]).
+MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
 <!-- Add more about the biology of this part here