Difference between revisions of "Part:BBa K624057"
| Line 4: | Line 4: | ||
pYMB essentials + RBS(Pmsp3) + LLO | pYMB essentials + RBS(Pmsp3) + LLO | ||
| − | pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 | + | pYMB is a shuttle vector for manipulating and transforming gene into AMB-1. |
RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter | RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter | ||
| − | of AMB-1 | + | of AMB-1 . |
LLO (Listeriolysin O) can make the bacteria be able to | LLO (Listeriolysin O) can make the bacteria be able to | ||
escape into the cytosol without damaging the plasma | escape into the cytosol without damaging the plasma | ||
Revision as of 11:21, 9 October 2011
pYMB essentials + RBS(Pmsp3) + LLO
pYMB essentials + RBS(Pmsp3) + LLO
pYMB is a shuttle vector for manipulating and transforming gene into AMB-1. RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 . LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. The ribosome binding site is from Pmsp3.(BBa_K624012).
The ribosome binding site is from Pmsp3.(BBa_K624012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2567
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
- 1000COMPATIBLE WITH RFC[1000]