Difference between revisions of "Part:BBa K624057"
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pYMB essentials + RBS(Pmsp3) + LLO | pYMB essentials + RBS(Pmsp3) + LLO | ||
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+ | pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 | ||
+ | RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter | ||
+ | of AMB-1 | ||
+ | LLO (Listeriolysin O) can make the bacteria be able to | ||
+ | escape into the cytosol without damaging the plasma | ||
+ | membrane, and grow intracellularly. | ||
+ | which help protect the AMB-1 from extracellular immune system. | ||
+ | The ribosome binding site is from Pmsp3.(BBa_K624012). | ||
The ribosome binding site is from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]). | The ribosome binding site is from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]). |
Revision as of 11:20, 9 October 2011
pYMB essentials + RBS(Pmsp3) + LLO
pYMB essentials + RBS(Pmsp3) + LLO
pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. The ribosome binding site is from Pmsp3.(BBa_K624012).
The ribosome binding site is from Pmsp3.(BBa_K624012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2567
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
- 1000COMPATIBLE WITH RFC[1000]