Difference between revisions of "Part:BBa K624056"
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pYMB essentials + RBS(Pmsp3) + minC | pYMB essentials + RBS(Pmsp3) + minC | ||
− | The ribosome binding site is from Pmsp3.( | + | pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 |
+ | RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter | ||
+ | of AMB-1 | ||
+ | minC,the division inhibitor,its overexpression | ||
+ | would lead to septation inhibition at all potential | ||
+ | division sites, a circumstance of filamentation. | ||
+ | The ribosome binding site is from Pmsp3.(BBa_K624012). | ||
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Revision as of 11:20, 9 October 2011
pYMB essentials + RBS(Pmsp3) + minC
pYMB essentials + RBS(Pmsp3) + minC
pYMB is a shuttle vector for manipulating and transforming gene into AMB-1 RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 minC,the division inhibitor,its overexpression would lead to septation inhibition at all potential division sites, a circumstance of filamentation. The ribosome binding site is from Pmsp3.(BBa_K624012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal AgeI site found at 1398 - 1000COMPATIBLE WITH RFC[1000]