Difference between revisions of "Part:BBa K624055"

Revision as of 11:19, 9 October 2011

pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

The Pmsp1 is the second strongest promoter on AMB-1. RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1 tetR serves as a switch,which can turn on the downstream gene when Tetracycline is present ;and vice versa. GFP serves as a reporter to show if the construct work or not. All of the ribosome binding sites are RBS from Pmsp3.(BBa_K624012).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2561

@@ Line 4: / Line 4: @@
 Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP
-All of the ribosome binding sites are RBS from Pmsp3.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624012 BBa_K624012]).
+The Pmsp1 is the second strongest promoter
+on AMB-1.
+RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter
+of AMB-1
+tetR serves as a switch,which can turn on the downstream gene
+when  Tetracycline is present ;and vice versa.
+GFP serves as a reporter to show if the construct work or not.
+All of the ribosome binding sites are RBS from Pmsp3.(BBa_K624012).
 <!-- Add more about the biology of this part here