Difference between revisions of "Part:BBa K624044"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K624044 short</partinfo>
 
<partinfo>BBa_K624044 short</partinfo>
  
 
ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP
 
ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP
 +
The ori serve as the Origin of Replication,and
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the Pmsp1 is the second strongest promoter
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on AMB-1.
 +
LLO (Listeriolysin O) can make the bacteria be able to
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escape into the cytosol without damaging the plasma
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membrane, and grow intracellularly.
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which help protect the AMB-1 from extracellular immune system.
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ECFP serves as a reporter to show if the construct work or not.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:17, 9 October 2011

pYMB essentials + RBS + LLO + RBS + Invasin + RBS + ECFP

ori + Pmsp1 + rep + RBS + LLO + RBS + Invasin + ECFP The ori serve as the Origin of Replication,and the Pmsp1 is the second strongest promoter on AMB-1. LLO (Listeriolysin O) can make the bacteria be able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. which help protect the AMB-1 from extracellular immune system. ECFP serves as a reporter to show if the construct work or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2567
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3573
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 4190
  • 1000
    COMPATIBLE WITH RFC[1000]