Difference between revisions of "Part:BBa K624027"

 
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<font size=3>pYMB(Fig. 1) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.</font>
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[[Image: PYMBWorkflow.jpg|center|frame|<center>Fig.1 pYMB Work Flow <br>The way we design the shuttle vector for magnetic bacteria</center>]]

Revision as of 09:39, 9 October 2011

pYMB (shuttle vector between Escherichia coli and Magnetospirillum magneticum AMB-1)

Shuttle vector between E. coli and Magnetospirillum magneticum AMB-1, containing [ori+Pmsp1+rep] (essentials of pYMB) on pUC19

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal PstI site found at 1173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1173
    Illegal NotI site found at 7
    Illegal NotI site found at 1166
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal PstI site found at 1173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1173
    Illegal NgoMIV site found at 920
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2504
    Illegal SapI site found at 1421


pYMB(Fig. 1) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone psB1A1with promotor Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

Fig.1 pYMB Work Flow
The way we design the shuttle vector for magnetic bacteria