Difference between revisions of "Part:BBa K624032"
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Facultative anaeroabic magnetotactic bacterial species, Magnetospirillum magneticum AMB-1. Grow in liquid MSGM (Magnetic Spirillum Growth Medium), or on MSGM agar plate.<font size=3>Like ''E. coli'', AMB-1 also grows in liquid medium, and this medium is called '''Magnetic Spirillum Growth Medium (MSGM)'''.  In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.</font>
 
Facultative anaeroabic magnetotactic bacterial species, Magnetospirillum magneticum AMB-1. Grow in liquid MSGM (Magnetic Spirillum Growth Medium), or on MSGM agar plate.<font size=3>Like ''E. coli'', AMB-1 also grows in liquid medium, and this medium is called '''Magnetic Spirillum Growth Medium (MSGM)'''.  In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.</font>
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<font size=3>'''MSGM's  ingredients'''
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<font color=red>(In grams per liter)</font>
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●Succinic acid            0.74 g
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●KH2PO4                    0.68 g
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●Sodium nitrate            0.12 g
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●Sodium thioglycolate      0.1 g
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●[http://2011.igem.org/File:Wolfe_vitamin.pdf *Wolfe's vitamin solution]  10 ml(stock)
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●[http://2011.igem.org/File:Wolfe.pdf *Wolfe’s mineral solution]  5  ml(stock)
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●Ferric quinate solution  2  ml(0.27 g of FeCl<font size=1>3</font> and 0.19 g of quinic acid in 100 ml water)
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  '''Adjust pH to 7.0 with NaOH'''
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'''NCBI:''' [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=19021 whole genome sequence]
 
'''NCBI:''' [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=19021 whole genome sequence]

Revision as of 07:16, 9 October 2011

Magnetospirillum magneticum AMB-1

Facultative anaeroabic magnetotactic bacterial species, Magnetospirillum magneticum AMB-1. Grow in liquid MSGM (Magnetic Spirillum Growth Medium), or on MSGM agar plate.Like E. coli, AMB-1 also grows in liquid medium, and this medium is called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.



MSGM's ingredients


(In grams per liter) 

●Succinic acid             0.74 g
●KH2PO4                    0.68 g
●Sodium nitrate            0.12 g
●Sodium thioglycolate      0.1 g
●[http://2011.igem.org/File:Wolfe_vitamin.pdf *Wolfe's vitamin solution]  10 ml(stock)
●[http://2011.igem.org/File:Wolfe.pdf *Wolfe’s mineral solution]  5  ml(stock)
●Ferric quinate solution   2  ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)
 Adjust pH to 7.0 with NaOH


NCBI: [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=19021 whole genome sequence]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]