Difference between revisions of "Part:BBa K539741"
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<partinfo>BBa_K539741 short</partinfo> | <partinfo>BBa_K539741 short</partinfo> | ||
| − | + | The coding sequence of kivd is from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. ''Kivd'' plays the vital rule in the isobutanol biosynthesis pathway. This part kivd is under TetR repressible promoter that constitutively ON and repressed by TetRcan. And terminator([https://parts.igem.org/Part:BBa_J61048 BBa_J61048]) is included. | |
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| + | Following is the isobutanol biosythesis pathway. | ||
| + | <br>https://static.igem.org/mediawiki/parts/b/bc/Pathway%28small%29.jpg | ||
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Revision as of 10:33, 8 October 2011
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)
The coding sequence of kivd is from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. Kivd plays the vital rule in the isobutanol biosynthesis pathway. This part kivd is under TetR repressible promoter that constitutively ON and repressed by TetRcan. And terminator(BBa_J61048) is included.
Following is the isobutanol biosythesis pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1735
- 1000COMPATIBLE WITH RFC[1000]