Difference between revisions of "Part:BBa K539713:Design"
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===Source=== | ===Source=== | ||
| − | + | The gene is cloned by Zeng lab in NCTU. | |
It comes from Bacillus subtilis. | It comes from Bacillus subtilis. | ||
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===References=== | ===References=== | ||
Revision as of 09:45, 8 October 2011
AlsS (alpha-acetolactate synthase protein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 308
Illegal BsaI.rc site found at 902
Design Notes
Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
Source
The gene is cloned by Zeng lab in NCTU. It comes from Bacillus subtilis.