Difference between revisions of "Part:BBa K539711:Design"
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===Source=== | ===Source=== | ||
| − | + | The gene is cloned by Zeng lab in NCTU. | |
It comes from Escherichia coli str. K-12 substr. MG1655 | It comes from Escherichia coli str. K-12 substr. MG1655 | ||
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===References=== | ===References=== | ||
Revision as of 09:34, 8 October 2011
ilvC (ketol-acid reductoisomerase NAD(P)-binding)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 592
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 178
Illegal BsaI.rc site found at 997
Design Notes
Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region, those RE site should not exist in the coding region.There are two EcoR1 site in original sequence of ilvC. Those RE site in the ilvC has been modified by point mutation.
Source
The gene is cloned by Zeng lab in NCTU. It comes from Escherichia coli str. K-12 substr. MG1655