Difference between revisions of "Part:BBa K539142:Design"

(New page: __NOTOC__ <partinfo>BBa_K539142 short</partinfo> <partinfo>BBa_K539142 SequenceAndFeatures</partinfo> ===Design Notes=== In our experiment, the intensity of RNA thermometer are °C, 42...)
 
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===Design Notes===
 
===Design Notes===
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In our experiment, the intensity of RNA thermometer are °C, 42°C, 32°C, 27°C.
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The promoter is not included, so everyone can add the interested promoter you want.
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With the help of RNA thermometer, we can control the color of pigment by different temperatures. When the temperature is lower than 37°C, the pigment is red because the translation of crtEBI. When the temperature is higher than 37°C, the pigment is orange because of the translation of crtY that convert lycopene to orange beta-carotene.
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===Source===
 
===Source===

Latest revision as of 07:29, 8 October 2011

crtEBI+RNA thermometer+crtY+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1974
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1510
    Illegal NgoMIV site found at 1640
    Illegal NgoMIV site found at 4611
    Illegal AgeI site found at 725
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter is not included, so everyone can add the interested promoter you want.

Source

Other parts come from parts-registry.

References