Difference between revisions of "Part:BBa K572100"
Line 1: Line 1:
 +
_NOTOC__
 +
<partinfo>BBa_K572100 short</partinfo>
 +
 +
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K572100 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K572100 parameters</partinfo>
 +
<!-- -->
 
<html>
 
<html>
 
<img src="https://static.igem.org/mediawiki/parts/3/3e/PSB1PR1.GIF" WIDTH="525PX" HEIGHT="525PX">
 
<img src="https://static.igem.org/mediawiki/parts/3/3e/PSB1PR1.GIF" WIDTH="525PX" HEIGHT="525PX">

Revision as of 04:08, 6 October 2011

_NOTOC__ Light based screening plasmid backbone - pSB1Pc

The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2139
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2139
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 2015
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2139
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2139
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2154
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.